| Microorganism name |
Corynebacterium glutamicum
|
| Target proteins |
Leghemoglobin (Wang et al., 2023)
|
| Wild-type or GMO |
GMO (Wang et al., 2023)
|
| Production mode (intracellular/extracellular) |
Intracellular (Wang et al., 2023)
|
| Protein yield (g/L or g/g?) |
Not reported in g/L, but leghemoglobin accounted for approximately 20% total protein produced by the cell (Wang et al., 2023)
|
| Temperature used in study |
30°C (Wang et al., 2023)
|
| pH used in study |
NA |
| C & N source |
Tryptone, yeast extract (LB medium) supplementred with glucose (Wang et al., 2023)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
No FDA GRAS approval in US, not allowed in Canada |
| Companies |
NA |
| Publications/references |
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Wang, M., Shi, Z., Gao, N., Zhou, Y., Ni, X., Chen, J., Liu, J., Zhou, W., Guo, X., Xin, B., Shen, Y., Wang, Y., Zheng, P., & Sun, J. (2023). Sustainable and high-level microbial production of plant hemoglobin in Corynebacterium glutamicum. Biotechnology for Biofuels and Bioproducts, 16(1). https://doi.org/10.1186/s13068-023-02337-9
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Haas, T., Graf, M., Nieß, A., Busche, T., Kalinowski, J., Blombach, B., & Takors, R. (2019). Identifying the Growth Modulon of Corynebacterium glutamicum. Frontiers in Microbiology, 10. https://doi.org/10.3389/fmicb.2019.00974
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Lee, M. J., & Kim, P. (2018). Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application. Frontiers in Microbiology, 9. https://doi.org/10.3389/fmicb.2018.02523
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Lee, S. M., & Jeong, K. J. (2022). Advances in Synthetic Biology Tools and Engineering of Corynebacterium glutamicum as a Platform Host for Recombinant Protein Production. Biotechnology and Bioprocess Engineering, 28(6), 962–976. https://doi.org/10.1007/s12257-022-0219-1
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Zhang, B., Jiang, Y., Li, Z., Wang, F., & Wu, X. (2020). Recent Progress on Chemical Production From Non-food Renewable Feedstocks Using Corynebacterium glutamicum. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.606047
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Bello, P. R., Anzola, A. G., Becerril, A. O., & Gutiérrez, D. F. (2025). Evaluation of the Potential of Corynebacterium glutamicum ATCC 21492 for L-Lysine Production Using Glucose Derived from Textile Waste. Fermentation, 11(6), 355. https://doi.org/10.3390/fermentation11060355
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Liu, X., Zhang, W., Zhao, Z., Dai, X., Yang, Y., & Bai, Z. (2016). Protein secretion inCorynebacterium glutamicum. Critical Reviews in Biotechnology, 37(4), 541–551. https://doi.org/10.1080/07388551.2016.1206059
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Eastham, J. L., & Leman, A. R. (2024). Precision fermentation for food proteins: ingredient innovations, bioprocess considerations, and outlook — a mini-review. Current Opinion in Food Science, 58, 101194. https://doi.org/10.1016/j.cofs.2024.101194
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Follmann, M., Ochrombel, I., Krämer, R., Trötschel, C., Poetsch, A., Rückert, C., Hüser, A., Persicke, M., Seiferling, D., Kalinowski, J., & Marin, K. (2009). Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis. BMC Genomics, 10(1). https://doi.org/10.1186/1471-2164-10-621
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